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Rapid ethanolysis procedure for jojoba wax analysis by gas liquid chromatography
Author(s) -
Duncan C. C.,
Yermanos D. M.,
Kumamoto J.,
Levesque C. S.
Publication year - 1974
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636023
Subject(s) - wax , chromatography , chemistry , anhydrous , reagent , gas chromatography , fatty alcohol , hydrolysis , fatty acid , ethyl ester , organic chemistry
A rapid ethanolysis procedure for preparing jojoba wax for gas liquid chromatographic analysis is described. The wax esters are hydrolyzed by refluxing 4 drops of jojoba wax in 5% HCl in anhydrous ethanol in a test tube. The resulting fatty acid ethyl esters and fatty alcohols are separated and quantitated by a single gas liquid chromatographic run. Analysis of duplicate samples by this procedure gives essentially the same results as a procedure which requires 10 times more sample and reagents and considerably more time.