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The effect of 2,4,6,8‐tetramethyldecanoic acid on the hydrolysis and acylation of phospholipid by lipase in isooctane
Author(s) -
Yamaoka Masakazu,
Fortkamp Jens,
Morr Michael,
Schmid Rolf D.
Publication year - 1992
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02635925
Subject(s) - lipase , lysophosphatidylcholine , hydrolysis , acylation , chemistry , phospholipid , fatty acid , chromatography , phosphatidylcholine , biochemistry , enzyme , catalysis , membrane
The effect of an unusual natural fatty acid, 2,4,6,8‐tetramethyldecanoic acid (TMDA), on the hydrolysis and acylation of phospholipid by lipase in isooctane was studied. Lipases examined were from Rhizopus delemar, Candida cylindracea and Pseudomonas sp. The lipase was dispersed in isooctane by dioleoylphosphatidylethanolamine (DOPE) with or without the fatty acid and decanoyl‐lysophosphatidylcholine (LPC). The effect of TMDA on both the hydrolysis of DOPE and the acylation of LPC was compared with that of decanoic acid (DA) by varying the water content. At the higher water content, the hydrolysis of DOPE was enhanced or trace amount of phosphatidylcholine was produced. Hydrolysis was enhanced more by the addition of TMDA than by DA. The effect of TMDA on the acylation of LPC was similar to that of DA.

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