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A sensitive spectrophotometric method for lipid hydroperoxide determination
Author(s) -
Løvaas Erik
Publication year - 1992
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02635914
Subject(s) - chromophore , iodide , chemistry , interference (communication) , catalysis , chromatography , spectrophotometry , photochemistry , inorganic chemistry , biochemistry , computer science , computer network , channel (broadcasting)
A spectrophotometric method for the quantitation of lipid hydroperoxides is described. The chromophore produced in the reaction is the strongly absorbing I 3 − ion, which has been used previously by many researchers. The present method deviates from earlier methods in three respects—first, the assay is done at low acid concentrations; second, the iodide concentration is reduced; and third, Fe 2+ is used as a catalyst. As a result of these modifications the interference from oxygen is minimized. The assay is fast, sensitive and simple to perform.