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Analysis of lipid classes by solid‐phase extraction and high‐performance size‐exclusion chromatography
Author(s) -
Hopia Anu I.,
Piironen Vieno I.,
Koivistoinen Pekka E.,
Hyvönen Lea E. T.
Publication year - 1992
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02635913
Subject(s) - chromatography , degree of unsaturation , solid phase extraction , size exclusion chromatography , chemistry , extraction (chemistry) , divinylbenzene , phase (matter) , sterol , phospholipid , gel permeation chromatography , styrene , organic chemistry , cholesterol , biochemistry , membrane , copolymer , enzyme , polymer
An improved method to analyze lipid classes of edible oils and fats by solid‐phase extraction (SPE) and high‐performance size‐exclusion chromatography (HPSEC) is presented. A mixture of lipid standards was fractionated by the solid‐phase extraction procedure (NH 2 phase) into polar and nonpolar fractions; these were then submitted to analysis by HPSEC. The size‐exclusion chromatographic columns were three styrene/divinylbenzene columns with pore sizes of 100 Å and 50 Å. Light‐scattering was used for the detection system, and the parameters of the detector were optimized to minimize the difference between the responses of the compounds studied. With this procedure it was possible to separate the following lipid classes: triacylglycerols, diacylglycerols, monoacylglycerols and free fatty acids, sterols, sterol esters, tocopherols and carotenoids. Quantitative analysis was studied for a light‐scattering detector with several lipid standards of different molecular weights and unsaturation levels.