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Faster and easier methods for quantitative lipid extraction and fractionation from miniature samples of animal tissues
Author(s) -
ElmerFrohlich Kathryn,
Lachance Paul A.
Publication year - 1992
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02635894
Subject(s) - chromatography , fractionation , extraction (chemistry) , chemistry , chloroform , methanol , organic chemistry
A less time‐consuming dry‐column method for total lipid extraction from tissue samples was scaled down and modified to permit the evaluation of 1‐g samples of liver and muscle tissues from rats and from obese (ob/ob) and lean mice. Lipid yields obtained by the new dry‐column method compared well with those obtained by the widely accepted traditional chloroform/methanol method. For subsequent separation of neutral and polar lipid classes, a solid‐phase fractionation method was developed. Its performance was verified by thin‐layer chromatogrphy. Both column chromatographic methods were found to be especially convenient when running multiple samples simultaneously.