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Automated determination of protease for laundry compounds
Author(s) -
Detmar D. A.,
Vogels R. J.
Publication year - 1971
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02635690
Subject(s) - autoanalyzer , laundry , chromatography , casein , protease , coefficient of variation , chemistry , correlation coefficient , acetic acid , analytical chemistry (journal) , sample (material) , substrate (aquarium) , mathematics , statistics , food science , biochemistry , enzyme , biology , waste management , engineering , ecology
Abstract For the determination of the activity of protease for laundry washing compounds preference is given to casein as substrate and measurement of the supernatant at 275 nm after precipitation with trichloro‐acetic acid. Since sampling rate and precision with the AutoAnalyzer are in this case very low, an automated system based on the principle of the mechanized test tube has been developed. The sampling rate is one sample per minute; the coefficient of variation of a single sample is 2.3% without relation to a standard solution and 1.6% if results are related to a standard solution run simultaneously. If the activity of a sample is determined in threefold, the coefficients of variation are 1.6% resp. 1.1%.