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Four‐factor response surface optimization of the enzymatic modification of triolein to structured lipids
Author(s) -
Shieh ChwenJen,
Akoh Casimir C.,
Koehler Philip E.
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02635645
Subject(s) - triolein , capric acid , substrate (aquarium) , chemistry , yield (engineering) , lipase , fatty acid , chromatography , hexane , response surface methodology , glyceride , molar ratio , enzyme , organic chemistry , materials science , catalysis , lauric acid , oceanography , geology , metallurgy
Abstract The ability of an immobilized lipase to modify the fatty acid composition of (88.8% C 18:1 , 4.3% C 16:0 , 3.1% C 18:0 , and 3.8% C 18:2 as determined by gas chromatography, and approximately 90% triolein) in hexane by incorporation of a medium‐chain fatty acid, capric acid (C 10 ), to form structured triacylglycerol was studied. Response surface methodology was used to evaluate the effect of synthesis variables, such as reaction time (12–36 h), temperature (25–65°C), molar substrate ratio of capric acid to triolein (2:1–6:1), and enzyme amount (10–30% wt% of triacylglycerol), on the yield of structured lipid. Optimization of the transesterification was attempted to obtain maximum yield of structured lipid while using the minimum molar substrate ratio and enzyme amount as much as possible. Computer‐generated contour plot interpretation revealed that a relatively high molar substrate ratio (6:1) combined with low enzyme amount (10%) after 30 h of reaction at 25°C gave optimum incorporation of capric acid. A total yield for combined monoand dicaproolein of up to 100% was obtained.