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Methods for the determination of cyclopropenoid fatty acids V. A spectrophotometric method for cottonseed oils based upon the Halphen‐test reaction
Author(s) -
Bailey A. V.,
Pittman R. A.,
Magne F. C.,
Skau E. L.
Publication year - 1965
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02635582
Subject(s) - cottonseed oil , titration , chemistry , chromatography , cottonseed , color reaction , relative standard deviation , fatty acid , organic chemistry , food science , reagent , detection limit
A spectrophotometric method of analysis for the quantitative estimation of cyclopropenoid fatty acids in cottonseed oil based upon the Halphen‐test reaction has been described. Various parameters involved in the reaction have been investigated and two pigment fractions responsible for the characteristic Halphen‐test cherry‐red color have been isolated. The method is applicable to relatively small amts of sample material. The average deviation from the actual cyclopropenoid acid contents as determined by the stepwise HBr titration method was less than ŷ0.02% in both the refined and crude oil series.