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Preparation of 14 C‐gossypol by incorporation of acetate‐1‐ 14 C and acetate‐2‐ 14 C by biosynthesis
Author(s) -
Smith F. H.
Publication year - 1974
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02635019
Subject(s) - gossypol , chemistry , biosynthesis , nuclear chemistry , germination , chromatography , organic chemistry , biochemistry , botany , biology , enzyme
Details for the germination of cotton seedlings and their utilization for incorporating 14 C‐labeled acetate into gossypol are presented. Methods for the isolation and purification of the biosynthesized gossypol are given. The specific activity of the gossypol obtained from root tissue and cotyledons had mean values for 8 preparations of 428.6×10 3 and 6.3×10 3 dpm/mg, respectively. Corresponding incorporations were 5.42 and 0.32%.