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Passive cutaneous anaphylaxis in the detection of seed antigens of Ricinus communis (castorbean)
Author(s) -
Layton Laurence L.,
Lee Stephen,
Dante Beatrice T.,
DeEds Floyd
Publication year - 1961
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02633367
Subject(s) - antigenicity , castor oil , antiserum , ricinus , antigen , meal , chemistry , anaphylaxis , chromatography , food science , biochemistry , biology , immunology , allergy
The phenomenon of passive cutaneous anaphylaxis in guinea pigs was shown to be applicable in the laboratory determination of residual antigenicity in fractionated and chemically treated castor‐seed proteins. Six protein fractions obtained by paper‐strip electrophoresis of castorseed protein were shown to be antigenic. Castor‐seed meal which had been cooked 20 minutes at 100ŶC. in Ca(OH) 2 solution at pH 12.4 was shown to retain some of its original antigenicity, while meal cooked for 32 minutes under the same conditions of pH and temperature would not elicit the P.C.A. reaction in guinea pigs sensitized with rabbit antiserum to castor‐seed protein.