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Automated bioassay of proteolytic enzymes in detergents
Author(s) -
Paixao L. M.,
Babulak S. W.,
Barkin S. M.,
Shumway D. K.,
Friedman S. D.
Publication year - 1969
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02633172
Subject(s) - proteases , proteolytic enzymes , casein , digestion (alchemy) , chemistry , bioassay , chromatography , amino acid , enzyme , biochemistry , quantitative analysis (chemistry) , biology , genetics
An automated biological assay for proteases in detergents was developed. The assay involved enzymic digestion of casein followed by the analysis of the liberated amino groups. Quantitative determination of the proteolytic activity was accomplished by reacting the amino groups with trinitrobenzene‐sulfonic acid (TNBS). An automated system was used to carry out both the digestion and the quantitative color development of the TNBS‐amino group complex. The method is accurate, reproducible, easy to handle, fast and free from interferences by any of the standard detergent ingredients. It may also be used as an investigative tool in enzyme research.