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Laboratory separation of crystals from plastic fats using detergent solution
Author(s) -
Poot C.,
Dijkshoorn W.,
Haighton A. J.,
Verburg C. C.
Publication year - 1975
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02633039
Subject(s) - fraction (chemistry) , aqueous solution , chromatography , dilution , chemistry , centrifugation , materials science , phase (matter) , organic chemistry , physics , thermodynamics
An improved laboratory technique for isolating the solid‐fat fraction from plastic fats is described. The fat first is dispersed in an aqueous detergent solution; then the oil and solid fat fractions are separated by high speed centrifugation. To facilitate the separation, a 25% ammonia solution is added to adjust the density of the water phase to between those of the oil and the solid‐fat phase. After rinsing with cold ethanol, a solid‐fat fraction is obtained‐contaminated with only ca. 5% oil‐which can directly be used for differential thermal and X‐ray analyses. The method also can be applied for a quick determination of the solid‐fat content according to the dye dilution principle and for obtaining oil‐free fat crystals for electron microscopy.