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Fluorometric measurement of aflatoxins
Author(s) -
Childs E. A.,
Ayres J. C.,
Koehler P. E.
Publication year - 1970
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02632968
Subject(s) - fluorometer , aflatoxin , chromatography , chemistry , filter paper , filter (signal processing) , fluorescence , analytical chemistry (journal) , food science , computer science , optics , physics , computer vision
An accurate, precise and sensitive fluorometric method for quantifying purified aflatoxins in solution is proposed. After purification, aflatoxin samples are read in a fluorometer with a primary filter of 365 nm and a secondary filter of 435 nm for B 1 and B 2 and 465 nm for G 1 and G 2 . The method is precise at as little as 0.0002 µg/ml B 1 and G 1 and 0.00006 µg/ml B 2 and G 2 , and accurate at concentrations of 0.002 µg/ml B 1 and G 1 and 0.0006 µg/ml B 2 and G 2 .