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Determination of structure of unsaturated fatty acids via reductive ozonolysis
Author(s) -
Privett O. S.,
Nickell Christense
Publication year - 1962
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02632864
Subject(s) - ozonolysis , chemistry , organic chemistry , pentane , chromatography , polyunsaturated fatty acid , acylation , linoleic acid , gas chromatography , fatty acid , catalysis
An improved method of reductive ozonolysis for the determination of structure of unsaturated fatty acids is reported. The ozonization is carried out at 蜢60舑蜢70C by adding the sample dissolved in pentane to a .02舑.03 M pentane solution of ozone. The reduction is effected by the Lindlar catalyst at 0C in pentane or in other solvents, such as the methyl esters of short‐chain fatty acids or in dimethyl phthalate, and the aldehydic fragments are analyzed by gas‐liquid chromatography (GLC). The method is applied to the structural analyses of methyl esters of oleic, linoleic, linolenic, and arachidonic acids. The sensitivity of the method is demonstrated by the analysis of methyl oleate containing a small amount of added methyl linolenate. Mixtures of methyl oleate and petroselinate are analyzed to demonstrate the identification of the simple aldehydes and ester‐aldehydes, and to show the potential of the method for quantitative analysis of mixtures of unsaturated esters.