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Incorporation of linolenic‐1‐C 14 acid into eicosapentaenoic and docosahexaenoic acids in fish
Author(s) -
Kayama Mitsu,
Tsuchiya Yasuhiko,
Nevenzel J. C.,
Fulco Armand,
Mead J. F.
Publication year - 1963
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02632846
Subject(s) - docosahexaenoic acid , eicosapentaenoic acid , docosapentaenoic acid , polyunsaturated fatty acid , linolenic acid , chemistry , palmitoleic acid , arachidic acid , biochemistry , chromatography , fatty acid , food science , linoleic acid , palmitic acid
Following intraperitoneal injection of methyl linolenate‐1‐C 14 into kelp bass, Paralablax clathratus , the highly polyunsaturated fatty acids of their body fats were concentrated by low temperature crystallization from acetone, and eicosapentaenoic and docosahexaenoic acids were isolated from the concentrate by reversed‐phase chromatography and hydrogenated. The resulting arachidic and behenic acids were degraded stepwise to margaric acid, and the distribution of activity was determined. The results indicate that the injected linolenic acid was converted to eicosapentaenoic acid and the latter incorporated into docosahexaenoic acid. A probable conversion pathway is linolenic acid薔6,9,12,15‐octadecatetraenoic acid薔8,11,14,17‐eicosatetraenoic acid薔5,8,11,14,17‐eicosapentaenoic acid薔7,10,13,16,‐19‐docosapentaenoic acid薔4,7,10,13,16,19‐docosahexaenoic acid.