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Preparation of highly purified fatty acids via liquid‐liquid partition chromatography
Author(s) -
Privett O. S.,
Nickell E. Christense
Publication year - 1963
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02632579
Subject(s) - chromatography , chemistry , fractionation , distillation , polyunsaturated fatty acid , linolenate , partition coefficient , silicic acid , elution , high performance liquid chromatography , fatty acid methyl ester , adsorption , organic chemistry , fatty acid , catalysis , biodiesel
The application of reversed‐phase, liquid‐liquid partition chromatography to the preparation of highly purified methyl esters of fatty acids is described. The parameters of fractionation of methyl esters by this method are demonstrated with model mixtures of these compounds. Model mixtures are also used to demonstrate the use of adsorption chromatography on columns of silicic acid, impregnated with silver nitrate, in conjunction with liquid‐liquid partition chromatography to eliminate fractional distillation in the preparation of polyunsaturated methyl esters. The formation of artefacts of 4, 5 and 6 double bond methyl esters during fractional distillation and their fractionation is described. The use of liquid‐liquid partition chromatography for preparative purposes on a large laboratory scale is demonstrated by the preparation of pure methyl linolenate from linseed oil esters. Methyl arachidonate, eicosapentaenoate, docosapentaenoate and docosahexaenoate are also prepared in high purity.