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Isolation of ricin, ricinine, and the allergenic fraction from castor seed pomace from two different sources
Author(s) -
Waller George R.,
Negi S. S.
Publication year - 1958
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02632557
Subject(s) - pomace , ricin , chemistry , fraction (chemistry) , food science , extraction (chemistry) , solvent extraction , botany , chromatography , biology , biochemistry , toxin
Summary A scheme of separation is described for the extraction of ricin, ricinine, and the allergenic fractions from the same lot of castor seeds, thereby considerably curtailing the hazard associated with the handling of the material. The yield of ricin from the commercially prepared pomace was only one‐tenth of that from the laboratory‐prepared pomace, which contained approximately 1% of this toxic protein. Likewise the amount of dialyzed allergenic fraction present in commercial pomace is only about one‐third of that present in the laboratory sample, which contained 0.22% of this constituent. The laboratory pomace contained five times as much (0.10%) of the alkaloid ricinine as did the commercial pomace. It appears that commercial processing of the pomace is effective in destroying a considerable portion of the toxic and allergenic activity in castor seeds. The pomace remaining from the extraction is nontoxic and may be used to provide a source of protein for feeding stuffs.