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Aflatoxins M 1 and M 2 : Preparation and purification
Author(s) -
Stubblefield R. D.,
Shan G. M.,
Shotwell O. L.
Publication year - 1970
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02632471
Subject(s) - aflatoxin , silicic acid , chloroform , chemistry , chromatography , elution , silica gel , acetonitrile , ethanol , fermentation , aspergillus flavus , fraction (chemistry) , organic chemistry , food science
A crude product containing aflatoxins M 1 and M 2 , as well as large quantities of aflatoxins B 1 and B 2 , obtained by fermentation of rice with Aspergillus flavus NRRL 3251 was chromatographed on a silicic acid column. Almost all the B 1 and B 2 were separated from M 1 and M 2 . Aflatoxins M 1 and M 2 were eluted together with ethanol‐chloroform (5∶95 v/v). The combined M 1 and M 2 fraction was placed on a Merck silica gel (0.05–0.2 mm) column to be washed with hexane‐chloroform (1∶1 v/v) and chloroform to remove traces of B 1 and B 2 and eluted with ethanol‐chloroform (1.5∶98.5 v/v) to obtain aflatoxin M 1 and mixtures of M 1 and M 2 . Rechromatography of M 1 on another silica gel column gave pure M 1 which was crystallized from acetonitrile. Aflatoxin M 2 was prepared by hydrogenation of M 1 and crystallized from acetonitrile.