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Comparative analytical studies of fatty acids of the alga Chlorella pyrenoidosa
Author(s) -
Schlenk Hermann,
Mangold H. K.,
Gellerman J. L.,
Link W. E.,
Morrissette R. A.,
Holman R. T.,
Hayes H.
Publication year - 1960
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02631594
Subject(s) - chlorella pyrenoidosa , autoxidation , chemistry , chromatography , isomerization , distillation , linoleic acid , organic chemistry , degree of unsaturation , gas chromatography , linolenic acid , fatty acid , chlorella , algae , catalysis , biology , botany
Gas‐liquid chromatography, several versions of paper chromatography, and alkaline isomerization have been applied to one preparation of Chlorella fatty acid methyl esters. GLC outranks other methods in rapidity, reproducibility, and resolving power. The presence of nonvolatile components in the sample can lead to erroneous results. Among PC methods which might arise from autoxidation and polymerization are detected. Esters or acids with 14 or fewer C atoms should be analyzed as nonvolatile derivatives. AI, as used here, introduces a systematic error in the determination of linoleic and linolenic acids. The quantitative results of the methods are in agreement for the major components, which all belong to the C 10 and C 18 series. A number of minor components have been revealed by GLC and/or PC. They are tentatively identified as