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Dietary antioxidants in young swine
Author(s) -
Hill E. G.
Publication year - 1963
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02631559
Subject(s) - hemolysis , vitamin e , tocopherol , autoxidation , polyunsaturated fatty acid , chemistry , antioxidant , thiobarbituric acid , ethoxyquin , food science , biochemistry , tocopheryl acetate , vitamin , lipid peroxidation , biology , fatty acid , immunology
Young swine obtained by hysterectomy were fed purified diets low in vitamin E and supplemented with d‐ॅ‐tocopheryl acetate and ethoxyquin (Santoquin R ). It was demonstrated that with very low levels of polyunsaturated fatty acids (PUFA) in the diet, both tocopherol and Santoquin protected the tissues of the pig from increased thiobarbituric acid (TBA) values and from increased hemolysis usually associated with low vitamin E status. When the dietary PUFA were increased to levels over 5%, the supplements of tocopherol and Santoquin protected against increased TBA values of tissue homogenates, but not against increased hemolysis of erythrocytes, even when blood serum showed substantial amounts of tocopherol. Some of the interrelationships of dietary PUFA and ॅ‐tocopherol were demonstrated. It was shown that for each 1% of peroxidized corn oil added to the diet above 4%, roughly 100 mg of d‐ॅ‐tocopheryl acetate was necessary to protect the pigs from erythrocyte hemolysis. The failure to reach a 舠zero舡 TBA value in vitamin E‐deficient swine tissue homogenates substantiated the theory of in vivo lipid autoxidation, and the increased TBA values of incubated tissue homogenates demonstrated in vitro lipid autoxidation in tissues not protected by a biological antioxidant.