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The application of gas‐liquid chromatography to the determination of vitamins E and K
Author(s) -
Nair P. P.,
Turner D. A.
Publication year - 1963
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02631557
Subject(s) - chromatography , gas chromatography , chemistry , tocopherol , chromatography column , trimethylsilyl , infrared spectrophotometry , ether , hydrophilic interaction chromatography , high performance liquid chromatography , column chromatography , polymer , vitamin e , organic chemistry , antioxidant
The separation and quantitation of vitamins E and K wave been made possible by gas‐liquid chromatography, using a mixture of two different silicone rubber polymers as the liquid stationary phase. ॅ‐Tocopherolquinone and ॅ‐Tocopherolhydroquinone could be separated from the original ॅ‐tocopherol by this technique. Crude lipid extracts from mammalian tissues contain an unknown component which converts ॅ‐tocopherolquinone to ॅ‐tocopherol during chromatography. The implications of this observation is discussed in the light of biological processes. A group of derivatives, with the trimethylsilyl ether link was prepared and characterized by gas chromatography and infrared spectrophotometry.