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Application of gas‐liquid partition chromatography to the quantitative estimation of monoglycerides
Author(s) -
McInnes A. G.,
Tattrie N. H.,
Kates M.
Publication year - 1960
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02630812
Subject(s) - chemistry , monoglyceride , anhydrous , chromatography , acetone , organic chemistry , gas chromatography , glyceride , pyridine , fatty acid
Summary A procedure has been developed for the quantitative estimation of monoglycerides in terms of their constituent fatty acids. The monoglyceride mixture is mesylated with mesyl chloride in the presence of pyridine, and the resulting dimesyl derivatives are converted to allyl esters of the constituent fatty acids by treatment with sodium iodide in anhydrous acetone at 100°C. The allyl esters are then analyzed quantitatively by gas‐liquid partition chromatography at 240°C. on a column of Apiezon M—Celite. Both α‐ and β‐monoglycerides are quantitatively converted to allyl esters by this procedure. β‐Monoglycerides in a mixture of α‐ and β‐isomers may be determined separately after removal of the α‐isomers by oxidation with periodic acid. The analytical procedure is also applicable to monoglycerides in the presence of free fatty acids, diglycerides, and triglycerides.