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Cocoa bean cell and embryo culture
Author(s) -
Wen MingChe,
German Bruce,
Kinsella John E.
Publication year - 1984
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02582135
Subject(s) - callus , suspension culture , agar , embryo , biology , botany , tissue culture , food science , composition (language) , cell culture , chemistry , biochemistry , microbiology and biotechnology , bacteria , in vitro , genetics , linguistics , philosophy
Callus culture of cocoa bean was initiated from immature cotyledons on agar medium. By dispersing these callus cells, a liquid suspension culture was established. The lipid composition of cocoa suspension culture was investigated and compared with those of cocoa beans of different maturities. Factors affecting fatty acid and triglyceride synthesis in cocoa suspension cultures also were studied. In parallel studies, asexual embryos were proliferated from immature zygotic embryos cultured in semisolid or liquid medium. The factors affecting the lipid composition in cultured embryos are discussed.