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Production of eicosapentaenoic acid by Saprolegnia sp. 28YTF‐1
Author(s) -
Shirasaka Norifumi,
Shimizu Sakayu
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02577852
Subject(s) - eicosapentaenoic acid , food science , polyunsaturated fatty acid , phosphatidylethanolamine , dry weight , arachidonic acid , chemistry , sucrose , yeast extract , phosphatidylcholine , fish oil , fatty acid , biology , biochemistry , phospholipid , botany , fermentation , fish <actinopterygii> , membrane , fishery , enzyme
Saprolegnia sp. 28YTF‐1, isolated from a freshwater sample, is a potent producer of 5,8,11,14,17‐ cis ‐eicosapentaenoic acid (EPA). The fungus used various kinds of carbon sources, such as starch, dextrin, sucrose, glucose, and olive oil for growth, and olive oil was the best carbon source for EPA production. The EPA content reached 17 mg/g dry mycelium (0.25 mg/L) when the fungus was grown in a medium that contained 2.5% olive oil and 0.5% yeast extract, at pH 6.0 and 28°C for 6 d with shaking. Accompanying production of arachidonic acid (AA; 3.2 mg/g dry mycelia, EPA/AA = 5.1) and other ω6 polyunsaturated fatty acids was low. Both EPA content and EPA/AA ratio increased in parallel by lowering growth temperature. Triglyceride was the major mycelial lipid ( ca. 84%), but EPA comprised only 2.2% of the total fatty acids of this lipid. About 40% of the EPA produced was found in polar lipids, such as phosphatidylethanolamine (EPA content, 28.2%), phosphatidylcholine (13.6%), and phosphatidylserine (21.2%).