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Improved high‐performance liquid chromatography of vitamin E vitamers on normal‐phase columns
Author(s) -
Shin TaiSun,
Godber J. Samuel
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02564244
Subject(s) - chromatography , acetic acid , chemistry , high performance liquid chromatography , acetone , methanol , phase (matter) , vitamin , column chromatography , reproducibility , biochemistry , organic chemistry
A method is described for improving column stability and reproducibility for analysis of vitamin E vitamers on normal‐phase high‐performance liquid chromatography columns with isooctane/ethyl acetate/acetic acid/2,2‐dimethoxypropane (98.5:0.9:0.85:0.1) mobile phase. Acetic acid in the mobile phase reduced retention times of vitamin E vitamer and increased column stability. A small concentration of 2,2‐dimethoxypropane, which reacts with water to form acetone and methanol, reduced the need for column regeneration and stabilized retention times.