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Gas‐liquid chromatography of hydroxy fatty esters: Comparison of trifluoroacetyl and trimethylsilyl derivatives
Author(s) -
Freedman Bernard
Publication year - 1967
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02558166
Subject(s) - trimethylsilyl , chemistry , gas chromatography , ether , organic chemistry , chromatography , derivative (finance) , resolution (logic) , artificial intelligence , computer science , financial economics , economics
Transformations of hydroxy to keto fatty esters have been monitored successfully by converting the hydroxy compounds to their trifluoroacetyl (TFA) or trimethylsilyl (TMS) derivatives followed by gas‐liquid chromatography (GLC). The TFA derivatives have shorter retention times, are better separated from the keto esters, and permit more complete resolution of saturated and unsaturated hydroxy fatty esters than the TMS derivatives. Improved methods for derivative preparation which are rapid and convenient are described. Methyl dimorphecolate (methyl 9‐hydroxy‐ trans,trans ,10,12‐octadecadienoate), which is thermally unstable and cannot be chromatographed as the trifluoroacetate or free hydroxy compound, was chromatographed satisfactorily as the trimethylsilyl ether.