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Fractionation of commercial frying oil samples using sep‐pak cartridges
Author(s) -
Sebedio J. L.,
Septier Ch.,
Grandgirard A.
Publication year - 1986
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02553080
Subject(s) - chromatography , chemistry , petroleum ether , fraction (chemistry) , elution , diethyl ether , fractionation , solvent , cartridge , tetrahydrofuran , silicic acid , acetic acid , extraction (chemistry) , organic chemistry , materials science , metallurgy
Commercial frying oil samples were fractionated by column chromatography on hydrated silicic acid according to the standardized DGF‐IUPAC‐AOAC method. The non‐polar fraction was isolated using a mixture of petroleum ether:diethyl ether (87:13), while the polar fraction was eluted by diethyl ether. These used frying oil samples were also fractionated using Sep‐Pak cartridges. The non‐polar fraction was eluted with 20 ml of a mixture of petroleum ether:diethyl ether (92:8), while the polar fraction was eluted with methanol. The purity of each fraction was studied by thin layer chromatography (TLC) and by the Iatroscan TLC/FID system using a mixture of hexane:tetrahydrofuran:acetic acid (97:3:1) as solvent system. The Sep‐Pak and the standardized methods gave similar results. This indicates that the state of degradation of a frying oil (detection of polar components) could be studied using Sep‐Pak cartridges, which is less time‐ and solvent‐consuming than column chromatography.