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High‐performance liquid chromatography of selected phenolic compounds in olive oils
Author(s) -
Akasbi M.,
Shoeman D. W.,
Csallany A. Saari
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02552708
Subject(s) - hydroxytyrosol , tyrosol , chromatography , chemistry , caffeic acid , acetic acid , high performance liquid chromatography , acetonitrile , ferulic acid , gradient elution , elution , phenols , phenolic acid , polyphenol , organic chemistry , antioxidant
A reverse‐phase high‐performance liquid chromatographic technique with isocratic elution has been developed to separate and quantitate the major phenolic compounds of the hydroalcoholic extracts of olive oils. Hydroxytyrosol, tyrosol, caffeic acid, p ‐hydroxyphenylacetic acid and homovanillic acid were analyzed on a μBonapak C18 column with an acetonitrile/water/acetic acid (20:90:0.18, vol/vol/vol) mixture as a mobile phase. Electrochemical detection provided selectivity as well as sensitivity. The method was applied to the analysis of the most important phenolic compounds in olive oils.