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Quantitative determination of monoglycerides and diglycerides by high‐performance liquid chromatography and evaporative light‐scattering detection
Author(s) -
Liu J.,
Lee T.,
Bobik E.,
GuzmanHarty M.,
Hastilow C.
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02552704
Subject(s) - glyceride , monoglyceride , chromatography , chemistry , chromatography detector , derivatization , diglyceride , high performance liquid chromatography , supercritical fluid chromatography , organic chemistry , fatty acid
Neutral lipid classes were separated with normal‐phase high‐performance liquid chromatography, and mono‐ and diglycerides were determined with an evaporative light‐scattering detector (ELSD). The 1,3‐diacylglycerols were resolved from the 1,2‐diacylglycerol positional isomers, although some 1,3‐diacylglycerols of low molecular weight interfered with the 1,2‐diacylglycerols of high molecular weight. For monoglycerides, the separations between 1‐(and 3‐)acyl and 2‐acylglycerols were optimized only between those pairs with identical fatty acyl groups. Samples were dissolved in a solvent mixture and analyzed without derivatization. The results (monoglyceride) obtained from this method agreed well with those derived from gas chromatographic and supercritical fluid chromatographic methods. The universal nature of the ELSD makes this method applicable to oils and emulsifiers containing both saturated and unsaturated fatty acyl moieties.