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Determination of total gossypol at parts‐per‐million levels
Author(s) -
Fisher Gordon S.,
Frank A. W.,
Cherry J. P.
Publication year - 1987
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02549298
Subject(s) - gossypol , cottonseed , residue (chemistry) , sample (material) , mathematics , chromatography , pulp and paper industry , chemistry , environmental science , food science , engineering , organic chemistry , biochemistry
The official AOCS method Ba 8‐78 for total gossypol has been modified by introduction of two clean‐up steps. These steps reduce sample blanks and also substantially increase the concentration of dianilinogossypol available for spectrophotometric measurement. An improved method of correcting sample absorptions for blanks, which reduces the possibility of over or under compensation, and criteria for judging the quality of the results have been added. With these modifications, the method is more than adequate for grading glandless cottonseed under Rule 112 of the National Cottonseed Products Association. As little as 1 ppm can easily be measured in sound cottonseed samples. The method is not universally applicable to mixtures that may contain TG, but the criteria of quality should permit identification of samples to which it is not applicable and, hence, prevent reporting of inaccurate TG concentrations. Additional modifications for use with foods and other complex mixtures are suggested.