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Adaptation of an HPLC system to quantify cholesterol
Author(s) -
Brown Helen G.
Publication year - 1987
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02546262
Subject(s) - cholesterol , high performance liquid chromatography , integrator , linear range , chromatography , linear relationship , chemistry , total cholesterol , adaptation (eye) , mathematics , biology , statistics , computer science , detection limit , biochemistry , computer network , bandwidth (computing) , neuroscience
An HPLC system for separating oxidized components of crude soybean oil was adapted for use in quantifying cholesterol. At 210 nm there was linear integrator repsonse to concentration of cholesterol. The response was linear at four detector ranges, and the relationship remained linear when data were adjusted to one range. Spray‐dried egg yolks contained 3,048 mg (± 117) of cholesterol per 100 g, a value similar to literature measures.