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Pseudomonas sp. lipase‐catalyzed synthesis of geranyl esters by transesterification
Author(s) -
Yee Lisa N.,
Akoh Casimir C.,
Phillips Robert S.
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02546220
Subject(s) - geranyl acetate , lipase , transesterification , chemistry , pseudomonas fluorescens , geraniol , organic chemistry , triacylglycerol lipase , pseudomonas , vinyl acetate , hexane , catalysis , immobilized enzyme , chromatography , enzyme , essential oil , bacteria , copolymer , polymer , biology , genetics
Pseudomonas sp. lipase was immobilized by adsorption onto five supports and tested for its ability to synthesize geranyl esters by transesterification using short‐chain triacylglycerols as acyl donors. Reaction mixtures were prepared in 2 mL of n ‐hexane, 0.1 M geraniol, 0.03M triacylglycerol, and 200 units of lipase, and incubated at 30°C and 200 rpm for 24 h. Overall, glass beads were the best support. Geranyl acetate and caproate performed best with Duolite (77.5 and 95.3%, respectively). Geranyl butyrate and caprylate performed best with polyvinylpyrrolidone, (80.2 and 95.5%, respectively). Values for nonimmobilized enzyme also were obtained. Immobilization improved yields, with geranyl caproate exhibiting best results.