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Enzymatic synthesis of oleyl oleate in dense fluids
Author(s) -
Knez Ž.,
Rižner V.,
Habulin M.,
Bauman D.
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02546210
Subject(s) - oleyl alcohol , supercritical fluid , lipase , chemistry , solvent , batch reactor , chromatography , catalysis , oleic acid , rhizopus oryzae , alcohol , organic chemistry , enzyme , biochemistry , fermentation
Esterification between oleic acid and oleyl alcohol, catalyzed by the Mucor miehei immobilized lipase in a batch‐stirred tank reactor with supercritical carbon dioxide as solvent produced higher reaction rates at supercritical conditions than in the solvent‐free system. A continuous fixed‐bed reactor was designed based on the results obtained from batch experiments. At 150 bar, 40°C, and with water activity 0.46% w/w, the activity of the enzyme preparation is practically unchanged when CO 2 was used as solvent. The addition of small amounts of water increases the conversion rate. The higher conversion also was observed at longer residence time. When n ‐butane was used as reaction medium, a decrease in conversion was observed.