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Preparation of therapeutic phospholipids through porcine pancreatic phospholipase A 2 ‐mediated esterification and lipozyme‐mediated acidolysis
Author(s) -
Hosokawa Masashi,
Takahashi Koretaro,
Kikuchi Yuji,
Hatano Mutsuo
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02546201
Subject(s) - lysophosphatidylcholine , phosphatidylcholine , hydrolysis , chemistry , phospholipase a1 , phospholipase , phospholipase a2 , chromatography , biochemistry , phospholipid , lipase , enzyme , membrane
Highly unsaturated fatty acid‐containing phospholipids (HUFA‐PL), which serve to increase the deformability of human red blood cells, were prepared through porcine phospholipase A 2 ‐mediated esterification of the lysophosphatidylcholine, which was derived from soy phosphatidylcholine (PC), and by Lipozyme‐mediated acidolysis. Through these processes, phospholipase A 2 , with formamide as a water mimic, enhanced the incorporation of HUFA into position sn ‐2 of PC and suppressed hydrolysis of the synthesized PL. On the other hand, Lipozyme‐mediated acidolysis between position sn ‐1 of soy PC and HUFA was enhanced by a combination of water and propylene glycol. Simultaneously, the recovered PL products showed decreased hydrolysis of newly synthesized health‐beneficial HUFA‐PL.