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Quantitative determination of medium chain triglycerides in infant formula by reverse phase HPLC
Author(s) -
Lee Theresa W.
Publication year - 1986
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02546036
Subject(s) - chromatography , acetonitrile , high performance liquid chromatography , acetone , chemistry , phase (matter) , solvent , chromatography detector , reversed phase chromatography , organic chemistry
Two methods were developed for the separation of medium chain triglycerides (MCT) using reverse phase HPLC. Both methods employed a C18 microbond HPLC column as the stationary phase and an isocratic solvent system. The first method described consists of acetonitrile/acetone as the mobile phase with a differential refractometer as the detector. In the second method, acetonitrile/water was used as the mobile phase and a UV detector at 210 nm. Trinonanoin was used as the internal standard for quantitative determination. This method is suitable for milk‐, whey‐ and soy protein‐based matrices. With minor modification, it is applicable to MCT levels ranging from 10 to 50% of total fat.