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Production of 9‐hydroperoxy‐γ‐linolenic acid by soybean lipoxygenase in a two‐phase system
Author(s) -
Hiruta Osamu,
Nakahara Toro,
Yokochi Toshihiro,
Kamisaka Yashushi,
Suzuki Osamu
Publication year - 1988
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02546005
Subject(s) - chemistry , lipoxygenase , substrate (aquarium) , linolenic acid , solubility , pulmonary surfactant , emulsion , chromatography , hydrolysis , aqueous two phase system , yield (engineering) , aqueous solution , enzyme , food science , organic chemistry , biochemistry , fatty acid , linoleic acid , biology , ecology , materials science , metallurgy
9‐Hydroperoxy‐γ‐linolenic acid (9‐GOOH) was produced selectively by soybean lipoxygenase (LG) from γ‐linolenic acid (GLA) using a two‐phase (borate buffer, pH 6.5/hexane) system at a low temperature (10°C) with some anionic surfactants that showed little inhibitory effect on the enzyme at pH 6.5. The system avoided the inhibitory effect of a higher substrate concentration and of hydroperoxide as well as low substrate solubility in an aqueous system. Not lipoxygenase‐2 (LG2) but lipoxygenase‐1 (LG1) was indicated to be responsible for the production of 9‐GOOH. Among the anionic surfactants examined, acetate and sarcosinate were shown to be suitable, but phosphate was not. Ca 2+ increased the 9‐GOOH productivity. The LG1 fraction gave the maximum yield of 35% with 0.5 mM Ca 2+ , ECT‐3N (anionic surfactant, acetate) at 10°C and at 4.8 mM GLA in an emulsion.