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Enrichment of phospholipids from neutral lipids in peanut oil by high‐performance liquid chromatography
Author(s) -
Singleton J. A.
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02545334
Subject(s) - chromatography , chemistry , hexane , peanut oil , adsorption , phospholipid , composition (language) , organic chemistry , biochemistry , membrane , raw material , linguistics , philosophy
Phospholipids from crude peanut oil were enriched on a 2‐cm silica column and subsequently separated from neutral lipids within the chromatographic system without prior concentration. Hexane effectively removed the bulk neutral lipids, leaving the adsorbed phospholipids on the silica precolumn. Individual phospholipids were separated from the remaining neutral lipids and from each other by using two mixed solvents and a gradient program. This method separates the phospholipids in approximately 27 min after the desired enrichment level has been reached.