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Transesterification of oil by fatty acid‐modified lipase
Author(s) -
Murakami Mototake,
Kawasaki Yoshihiro,
Kawanari Masami,
Okai Hideo
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02545321
Subject(s) - lipase , transesterification , chemistry , organic chemistry , fatty acid , triacylglycerol lipase , hydrolysis , interesterified fat , solvent , enzyme , catalysis
Fatty acid was covalently attached to lipase (EC 3.1.1.3.) from Phycomyces nites , yielding a modified lipase of higher specific activity in hydrolytic and synthetic reactions in organic solvents. Attached long‐chain fatty acids solubilized the lipase in organic solvent and, therefore, promotion of dispersibility in organic solvent resulted in much higher reactivity. The initial rate of transesterification by modified lipase was almost 40 times that of native lipase in organic solvent. The specificities and selectivity of the modified lipase depended on the kind of attached fatty acid.