Premium
The isolation and recovery of fatty acids with Δ5 unsaturation from meadowfoam oil by lipase‐catalyzed hydrolysis and esterification
Author(s) -
Hayes Douglas G.,
Kleiman Robert
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02545318
Subject(s) - lipase , degree of unsaturation , lipolysis , hydrolysis , chemistry , fatty acid , triacylglycerol lipase , polyunsaturated fatty acid , enzyme , biochemistry , organic chemistry , adipose tissue
This report examines the use of lipases for isolating fatty acids with Δ5 unsaturation from the seed oil of Limnanthes alba , or meadowfoam. Seven lipase types and three enzyme configurations (immobilized, “free” and reversemicellar encapsulated) were examined. All lipases discriminated against Δ5 acids to varying degrees, but the degree of discrimination was independent of enzyme configuration. Lipase‐catalyzed esterification of meadowfoam oil’s free fatty acids was much more successful for isolating Δ5 acyl groups than was lipolysis. For example, esterification directed by Chromobacterium viscosum lipase yielded a free fatty acid product containing >95% of the Δ5 acyl groups at >99% purity.