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Quantitative analysis of synthetic mixtures of triacylglycerols with fatty acids from caprylic to stearic
Author(s) -
Fabien R.,
Craske J. D.,
Wootton M.
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02545317
Subject(s) - chromatography , chemistry , interesterified fat , column chromatography , stearic acid , caprylic acid , fatty acid , organic chemistry , lipase , enzyme
Separation and quantitation of triacylglycerols tricaprylin to tristearin was achieved by high‐performance liquid chromatography with refractive index detection and an eluent composed of propionitrile and butyronitrile (80:20, vol/vol). Peak identification was based on the logarithms of retention times, and quantitation was achieved by way of theoretical relative response factors. The validity of the response factor calculations was tested by analysis of primary standard mixtures of saturated triacylglycerols and of triacylglycerols obtained by interesterification of binary mixtures of monoacid triacylglycerols.