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Enzymatic pretreatment of deodorizer distillate for concentration of sterols and tocopherols
Author(s) -
Ramamurthi Suresh,
McCurdy Alan R.
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02545310
Subject(s) - chemistry , lipase , distillation , chromatography , oleic acid , organic chemistry , fatty acid , tocopherol , sterol , methanol , polyunsaturated fatty acid , residue (chemistry) , vacuum distillation , triacylglycerol lipase , antioxidant , enzyme , biochemistry , cholesterol , vitamin e
Separation of sterols and tocopherols from fatty acids in deodorizer distillate was facilitated through lipase‐catalyzed modification of fatty acids in canola, mixed and soya deodorizer distillates. The fatty acid esterification with methanol catalyzed by SP‐382 (an immobilized nonspecific lipase) proceeded rapidly, with conversion of fatty acid to methyl ester in 5 h being 96.5, 83.5 and 89.4%, respectively. A model mixture of pure oleic acid and dl‐α‐tocopherol was used to study any potential side reactions that may lower the tocopherol content during the esterification reaction. Under the conditions employed, the loss of tocopherol was less than 5%. Simple vacuum distillation (1–2 mm Hg) was employed to remove the volatile fraction (methyl esters of fatty acids, some fatty acids and other volatiles) of the esterified deodorizer distillate, leaving behind sterols, sterol esters and tocopherols. Sterols and tocopherols were almost completely retained in the residue fraction with recoveries in the range of 95%. Overall recoveries of sterols and tocopherols after esterification and distillation were over 90% for all the deodorizer distillate samples.