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Proton nuclear magnetic resonance rapid and structure‐specific determination of ω ‐3 polyunsaturated fatty acids in fish lipids
Author(s) -
Sacchi Raffaele,
Medina Isabel,
Aubourg Santiago P.,
Addeo Francesco,
Paolillo Livio
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02545299
Subject(s) - polyunsaturated fatty acid , chemistry , nuclear magnetic resonance spectroscopy , tuna , fish <actinopterygii> , resonance (particle physics) , chromatography , fatty acid , food science , organic chemistry , fishery , biology , physics , particle physics
Based on proton nuclear magnetic resonance ( 1 H‐NMR) spectroscopy, a rapid and structure‐specific method for the determination of ω‐3 polyunsaturated fatty acids (PUFAs) in fish lipids is presented. The different chemical shift observed for the methyl resonance of ω‐3 PUFAs (δ=0.95 ppm) with respect to the methyl resonance of all other fatty acids (δ=0.86 ppm) has provided the possibility of proposing a new and rapid method for the determination of ω‐3 PUFA content. Twenty‐four fish lipid samples (raw, cooked and canned albacore tuna) produced results that showed good agreement between 1 H‐NMR analysis and gas chromatographic determination. Raw and cooked samples showed significantly higher levels of ω‐3 PUFA than canned tuna.