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Determination of triglyceride structure via silver nitrate‐TLC
Author(s) -
Blank M. L.,
Verdino B.,
Privett O. S.
Publication year - 1965
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02545035
Subject(s) - triglyceride , chemistry , silicic acid , chromatography , thin layer chromatography , fatty acid , lipase , hydrolysis , silver nitrate , organic chemistry , biochemistry , enzyme , cholesterol
A procedure for the quantitative analysis of component triglycerides by thin‐layer chromatography (TLC) with silver nitrate‐silicic acid coated plates in combination with lipase hydrolysis is described. The method is demonstrated on synthetic triglyceride mixtures and applied to several natural fats. The results indicate that the fatty acids of natural fats are not distributed among the triglycerides in accordance with any simple mathematical formula and methods based on mathematical distribution patterns cannot be generally applied.