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Conditions and techniques for thin layer chromatography of aflatoxins
Author(s) -
Nesheim Stanley
Publication year - 1969
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02545017
Subject(s) - silica gel , adsorption , chromatography , resolution (logic) , aflatoxin , thin layer chromatography , layer (electronics) , sulfate , chemistry , solvent , moisture , particle size , materials science , organic chemistry , food science , artificial intelligence , computer science
Satisfactory resolution of the four common aflatoxins, B 1 , B 2 , G 1 and G 2 , on thin layer chromatograms has been a recurring problem. The most frequently observed cause of poor resolution and tailing of spots in the chromatograms was the variable properties of the commercial silica gel‐calcium sulfate adsorbent preparations. Variations in quality were observed even from one container to the next within single lots produced by individual manufacturers. Other variables which affected the chromatography to some degree included adsorbent particle size, concentration and nature of the calcium sulfate binder, silica gel layer thickness and moisture content, vapor phase composition in the developing chamber and the solvent used for development.