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Phospholipid content and fatty acid composition of human heart
Author(s) -
Rocquelin G.,
Guenot L.,
Astorg P. O.,
David M.
Publication year - 1989
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02544583
Subject(s) - phospholipid , phosphatidylethanolamine , phosphatidylcholine , sphingomyelin , palmitic acid , chemistry , fatty acid , chromatography , stearic acid , composition (language) , biochemistry , clinical chemistry , organic chemistry , cholesterol , membrane , philosophy , linguistics
Phospholipid content and fatty acid composition of human heart were determined on 36 biopsy specimens collected during open heart surgery. The main phospholipid classes, phosphatidylcholine (PC), phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), and sphingomyelin (SPH) were separated by HPLC, quantified, and converted to fatty acid methyl esters which were chromatographed on capillary GLC columns. Sex and age (mainly 40–70) of patients had no significant influence on the relative distribution of phospholipid classes and only a slight effect on fatty acid composition. Incorporation of trans 18∶1 in phospholipid classes was low. cis and trans octadecenoic isomers seemed to be selectively incorporated, the Δ9 and Δ11 cis or trans isomers being predominant. Human and rat data were compared, and some species differences were noticed. In human PC, palmitic acid is higher and stearic acid much lower than in rat PC. Saturated dimethyl acetals (16∶0 and 18∶0) in PC and PE were greater for humans. Incorporation of 20∶4 n−6 in human PE is higher than in rat PE.