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Rapid transesterification and mass spectrometric approach to seed oil analysis
Author(s) -
Ayorinde Folahan O.,
Clifton James,
Afolabi Oladapo A.,
Shepard Robert L.
Publication year - 1988
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02544516
Subject(s) - chemistry , sodium methoxide , linoleic acid , transesterification , stearic acid , palmitic acid , oleic acid , chromatography , linolenic acid , mass spectrometry , fatty acid , food science , organic chemistry , methanol , biochemistry
Fatty acid composition of seed oil is determined in less than one hr using a quantitative one‐vial technique. The method of analysis requires alcoholic solutions of sodium methoxide mild enough for epoxy and polyunsaturated oils. Separation and characterization of component fatty acids were accomplished by high resolution gas chromatography‐mass spectrometry. Using this method, Vernonia galamensis seed oil is shown to contain 79–80% vernolic ( cis ‐12,13‐epoxy‐ cis ‐9‐octadecenoic) acid. Amaranthus cruentus , a West African vegetable crop, is shown to contain 17.3% palmitic acid, 3.2% stearic acid, 22.7% oleic acid, 54.7% linoleic acid and 2.1% linolenic acid.