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Existence of cholinephosphotransferase in mitochondria and microsomes of liver and lung of guinea pig and rat
Author(s) -
Ghosh Sukla,
Mukherjee Shyamali,
Das Salil K.
Publication year - 1990
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02544391
Subject(s) - lipidology , microsome , guinea pig , clinical chemistry , mitochondrion , chemistry , medicine , biochemistry , endocrinology , biology , in vitro
We reported earlier on the occurrence of cholinephosphotransferase in the mitochondria of guinea pig lung. In order to determine whether organ and/or species specificities exist in regard to the cholinephosphotransferase activity in mitochondria, we have compared the subcellular distribution of the enzyme in the liver and lungs of rats and guinea pigs. Even though the activity of the enzyme was higher in microsomes than it was in mitochondria, the mitochondrial activity was authentic in both tissues of both species. The authenticity of mitochondrial activity was established by marker enzyme studies and ultrastructural examination of mitochondrial preparations.