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LCAT inhibitors interfere with the enzymatic determination of cholesterol and triglycerides
Author(s) -
Harris William S.,
Rayford Alan
Publication year - 1990
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02544344
Subject(s) - dtnb , chemistry , triglyceride , cholesterol , sterol o acyltransferase , enzyme , lecithin , glycerol , clinical chemistry , biochemistry , lecithin—cholesterol acyltransferase , endocrinology , medicine , apolipoprotein b , lipoprotein , biology , glutathione
5,5‐Dithio bis ‐(2‐nitrobenzoic acid) (DTNB) and p ‐chloromercuriphenylsulfonic acid (PCMPS) are well‐known sulfhydryl inhibitors that are used to inhibit lecithin: cholesterol acyltransferase (LCAT). They were each found to interfere with the enzymatic assays of cholesterol and triglycerides. DTNB falsely reduced the measured plasma cholesterol content, and falsely increased triglyceride readings. The inteference with the triglyceride assay could be largely prevented by blanking for glycerol. PCMPS had only a slight effect on the cholesterol assay, but falsely lowered the triglyceride readings to a great extent, even with glycerol‐blanking. Thus, these inhibitors should be avoided when plasma samples are to be enzymatically analyzed for cholesterol or triglycerides.