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Albumin‐bound docosahexaenoic acid and collagen‐induced human platelet reactivity
Author(s) -
Gaudette Douglas C.,
Holub Bruce J.
Publication year - 1990
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02544332
Subject(s) - docosahexaenoic acid , arachidonic acid , chemistry , phosphatidic acid , polyunsaturated fatty acid , platelet , albumin , medicine , eicosapentaenoic acid , endocrinology , fish oil , biochemistry , fatty acid , platelet activation , thromboxane , clinical chemistry , biology , phospholipid , enzyme , membrane , fishery , fish <actinopterygii>
Abstract An in vitro system designed to mimic the effect of various plasma nonesterified (polyunsaturated) fatty acids on platelet function and metabolism was employed. Human platelet aggregation induced by submaximal (1.8 μg/ml) collagen stimulation was significantly inhibited by 2 min preincubation with 20 μM albumin‐bound docosahexaenoic acid (22∶6n−3) (DHA), but not by the other fatty acids tested. [ 3 H]Phosphatidic acid (PA) formation, an indicator of phospholipase C activation following platelet stimulation, was moderately inhibited by eicosapentaenoic acid (20∶5n−3), 11,14,17‐eicosatrienoic acid (20∶3n−3), dihomo‐γ‐linolenic acid (20∶3n−6), as well as DHA, but not by arachidonic acid (20∶4n−6); this inhibition of phospholipase C activation could not explain the differential effect of DHA on platelet aggregation. The decreased production of thromboxane A 2 (TxA 2 ), as assessed by [ 3 H]12‐hydroxy‐5,8,10‐heptadecatrienoic acid (HHT) formation, may account for the inhibition of collagen‐induced aggregation by 20 μM DHA. Surprisingly, preincubation with 40 μM albumin‐bound DHA, even though resulting in greater inhibition of collagen‐induced aggregation, had less impact on HHT formation. A small but significant increase in [ 3 H]prostaglandin D 2 (PGD 2 ) levels following 3‐min collagen stimulation may have contributed to the greater antiaggregatory effect of 40 μM DHA. It is concluded that increased plasma nonesterified DHA may contribute to the dampened platelet activation and altered metabolism following fish oil supplementation of the diet.

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