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A method for the simultaneous analysis of unconjugated and glycine‐conjugated bile acids by capillary gas‐liquid chromatography
Author(s) -
Iida Takashi,
Itoh Takako,
Hagiwara Kayoko,
Chang Frederic C.,
Goto Junichi,
Nambara Toshio
Publication year - 1989
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02544079
Subject(s) - glycine , chromatography , chemistry , conjugated system , capillary action , gas chromatography , ether , organic chemistry , amino acid , biochemistry , materials science , polymer , composite material
A method for the simultaneous analysis of unconjugated and glycine‐conjugated bile acids by means of capillary gas‐liquid chromatography without need for prior deconjugation is described. The method involves: i) the use of an aluminum‐clad fused‐silica capillary column coated with a very thin film (0.1 μm) of a highly thermostable bonded and crosslinked methyl polysiloxane, and ii) the analysis of the bile acids as their methyl ester‐dimethylethylsilyl ether derivatives. This method, used to separate the major free and glycine‐conjugated bile acids from human gall bladder bile, should be applicable for the analysis of other biological fluids.